The Method of Enzyme-Gravimetric Determination of Dietary Fiber Content and Its Advantages and Disadvantages

Enzyme-gravimetric method for determination of dietary fiber content and analysis of pros and cons Buckwheat is one of the major food products of people and is native to northern China. Buckwheat contains a wealth of protein and various trace elements, widely welcomed by the majority of users at the same time for the diabetic and hyperlipidemia who have a great help, and buckwheat contains a wealth of fiber content, you can use the crude fiber analyzer To measure crude fiber in buckwheat, it can also be measured using enzyme-gravimetry.
The principle of the enzyme-gravimetric method for determining dietary fiber is that the sample is thermally digested with α-amylase, protease, and glucosidase, respectively, to degrade macromolecular proteins and starches into small molecules. After enzymolysis, the sample solution was precipitated with ethanol, and the small molecule substance was dissolved in ethanol. The dietary fiber that had not been digested was precipitated in ethanol. The residue was obtained by filtration. The residue was washed with ethanol and acetone and weighed dry to obtain the total dietary fiber. content. The determination of insoluble dietary fiber and soluble dietary fiber is performed by direct filtration of the sample after enzymatic hydrolysis to obtain residues and filtrate. The dietary fiber enzyme-gravimetric assay is a method jointly recognized by the AOAC and the American Association of Cereal Chemists (AACC) established by 43 laboratories in 29 countries. It can not only measure foods. The total dietary fiber content in the. In China, hand-washing was used to determine the fiber content in the diet at the very beginning. However, due to the large error in the hand-washing method, the enzyme labeling method was used to measure the fiber content.
The enzyme-gravimetric method is used to measure the dietary fiber in various plants. The accuracy of the measured results is relatively high, and the same as the result of the determination using the crude fiber analyzer is in conformity with the requirement of the fiber content in the national standard. A better way to measure.

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