Crude protein analyzer is one of the instruments for measuring wheat protein

Wheat, one of the important crops, has its economic value greatly affected by quality differences. One of the important indexes for measuring the quality of wheat processing is the content of protein. Generally Kjeldahl method with high accuracy and precision can be used to measure the quality of wheat. However, this method is labor- and time-consuming, and the strong acid and alkali of the experimental product are also Has a serious impact on the environment. The Kjeldahl method, near-infrared spectroscopy, and Coomassie brilliant blue staining method were used to determine the protein content of wheat grain, to compare their measurement accuracy, determine their applicable time and range, and to explore an effective method for early determination of material quality in wheat breeding. . The Kjeldahl method can be used with crude protein analyzers, which are developed on the basis of this principle.

All three methods can determine the total protein content of the material. Crude protein analyzer Kjeldahl and near-infrared spectroscopy in the 95% confidence interval significance coefficient are less than 0.05, indicating that there is a linear relationship between these two methods and the annual average. Kjeldahl and near-infrared spectroscopy in the T test results, the significance of the probability is greater than 0.05, indicating that there is no significant difference between the protein content measured by these two methods and the average year, can be displayed well The protein level of the sample was measured. In the test results, the correlation between the Coomassie brilliant blue staining method and the annual average is not strong. This may be due to operational errors. It has been shown through experiments that this method is accurate only when there are trace amounts of protein.

Crude protein analyzer Kjeldahl test results are accurate, but the required equipment for the test are mostly special glass equipment, easy to damage, distillation and absorption device is complex, inconvenient to operate, the test process takes a long time, generally requires 7} 8h, the operation is cumbersome, the consumption of energy and reagents is large, and a large number of toxic gases are emitted during digestion, especially the grinding materials are required during the test. This method is not applicable to the determination of the quality of precious samples in the early stage of breeding.

DA7200 near-infrared spectroscopy is fast, only needs 10}20min at a time, high analysis efficiency, wide range of applicable samples, no need of pretreatment and small amount of sample, 3040 seeds can be used for analysis, low analysis cost, test reproduction Good performance, no damage to the sample, it is very cost-effective to identify and screen a large number of intermediate materials in the early stage of breeding. Coomassie brilliant blue staining is rapid and sensitive, but only when the determination of microproteins results are accurate, so the sample should be diluted to a protein concentration of 0.1} 1m}/ml before the test, and the test must be completed within 1h, otherwise testing The result is inaccurate [mo.

Through experiments, we have a certain understanding of the principles of the three methods and have a more in-depth study of the principles of the application of the crude protein analyzer. This has a good guiding role in the determination of proteins, and is suitable for practical work. The analysis method has important guiding significance.

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