Analysis of the Application of High-throughput Tissue Grinding Apparatus

During the research process, the grinding technology for the sample is also continuously improving. If the traditional grinding method is used for the electric glass homogenizer, only the same sample can be ground at a time, and the speed is slow, and each time After the sample is milled, the instrument must be disinfected and cleaned, which is troublesome. For the shortcomings of traditional grinding methods, the high-throughput tissue grinder introduced by our company can grind 2×24, 2×48, and 4×96 samples in one minute. Save time and effort, batch, small differences within the batch. Extracted proteins have higher specific activity and longer fragments of nucleic acid.

High-throughput tissue grinders can be dry milled, wet milled, mixed, and homogenized. The sample types processed include plant tissues, cells, bacteria, and yeasts. For the calculation of large flux samples, the grinding efficiency and quality can be greatly improved. In biomedicine, forensics, public security criminal investigation, agriculture, geology, chemical industry, RoHS, toys, environment, quality inspection, colleges and universities have a wide range of applications. The working principle of high-throughput tissue grinder is: with the sample The grinding ball is added to the centrifugal tube, and the adapter performs arc-type radial vibration in the horizontal direction. Under the action of high-frequency vibration, the grinding ball hits the sample at high speed to complete the grinding of the sample.

The number and size of grinding balls put into different samples varies, and the friction between the ball and the sample can cause the tissue or cells to break. High-throughput tissue grinder operation steps: 1. Add the processed sample and grinding ball to the centrifuge tube in turn; 2. Place the sampled centrifuge tube into the adapter; 3. Put the adapter with the sample into the solution. Frozen in nitrogen for several minutes; 4. The frozen sample and adapter in the middle of the fixture, adjust the position; 5. The fixture on the sample stage, positioning slot positioning, tighten the vertical positioning knob, safety lock home; 6 Cover the instrument's safety door; 7. Set the vibration frequency, vibration time; 8. Start the instrument, perform the crushing process; 9. Wait for the instrument to completely stop vibrating, shut down, open the safety door, remove the sample; 10. The instrument is homed. Note: 1. The instrument should be placed in a dry and ventilated environment; 2. The sample is symmetrically distributed in the sample holder to ensure that the sample holder is balanced; 3. Before the safety door is closed, it must be confirmed that the fixture has been completely fixed, no loose phenomenon; Do not exceed the maximum range of 2000 times/min when setting the oscillation frequency. 5. When freezing the sample, care should be taken to prevent liquid nitrogen from spilling and causing frostbite.

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