Method for measuring chlorophyll content of paste-like objects

Chlorophyll Content Determination Method for Paste Objects Chlorophyll is an important food pigment, which is classified into chlorophyll a and chlorophyll b. Its basic structure is a magnesium-bound porphyrin ring. Chlorophyll meter can be used to measure the chlorophyll content in the leaves of plants, but for the determination of the solution chlorophyll content can not use the chlorophyll meter, and the cream chlorophyll extracted from the leaves of plants is important for the preparation of various chlorophyll derivatives It is of great significance to establish an effective analytical method to determine the content of the intermediate, and the chlorophyll content of the paste cannot be measured using a chlorophyll meter. At this time, it is necessary to select other methods for measurement, and it is possible to use the atomic absorption Spectrometer to perform accurate measurements.
The paste-like chlorophyll is mainly sodium magnesium chlorophyllin. During the test, 0.100 5 g of sodium chlorophyllin is weighed and formulated into a stock solution of 50.00 mL of chlorophyll. Accurately transfer 1.00 mL sodium chlorophyllin solution to 8 50.00 mL volumetric flasks, and then add 0.00, 2.00, 2.20, 2.40, 2.60, 2.80, 3.00, 3.20 mL of 50.00 mg·L-1Mg2+ standard solution respectively, to constant volume. Shake well. Click to determine the environment requirements using the atomic absorption spectrophotometer to measure and draw the working curve, and calculate the linear regression equation. This linear equation can be used to calculate the chlorophyll content.

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